先決定:我們要從哪一種檢體找答案?
依研究目的選擇汗液、呼氣冷凝液或其他檢體,規劃採集方式、樣本量與前處理需求。
Choose sweat, exhaled breath condensate or another specimen, then plan collection, sample volume and preparation around the research question.
百洑傳息BIOFLUMEN & XENSOR LAB張鈞豪博士 · Dr. Chun-Hao Chang
微流體 · 生物感測 · 體外診斷MICROFLUIDICS · BIOSENSING · IVD
NYCU · MICROFLUIDICS & BIOSENSING
張鈞豪博士Dr. Chun-Hao Chang · Assistant Professor
從微尺度流動,
解讀生命訊息。
Decoding life’s messages through micro-scale flows.

EXPLORE · TOUCH · DISCOVER
依研究目的選擇汗液、呼氣冷凝液或其他檢體,規劃採集方式、樣本量與前處理需求。
Choose sweat, exhaled breath condensate or another specimen, then plan collection, sample volume and preparation around the research question.
以流道幾何與流動控制安排輸送、混合或分離,讓檢體在指定位置與試劑接觸;實際設計依檢測任務而定。
Channel geometry and flow control organize transport, mixing or separation, bringing the sample and reagents together where needed.
結合生物辨識與訊號轉換,觀察目標造成的電化學或光學變化。圖中的電極是感測方式的概念例子。
Biological recognition and signal transduction connect the target to an electrochemical or optical change. The illustrated electrodes are one conceptual example.
讀取訊號後,進行校正、對照與效能驗證,評估它能回答哪些研究問題。圖中曲線僅示意讀值流程。
Readout is followed by calibration, controls and performance validation to assess which questions the data can answer. The curve is illustrative.
流程概念示意;圖形與動畫未呈現實際裝置尺寸、流速或量測數據。Conceptual workflow; geometry, motion and signals do not represent measured results.
01 / ABOUT THE LAB
BIO + FLUMEN
CROSS-DISCIPLINARY + SENSOR
RESEARCH APPROACH
02 / RESEARCH STORIES




01 / WEARABLE SAMPLING
Chemical Engineering Journal · 499, 155994
02 / SAMPLE PRETREATMENT
Sensors and Actuators B: Chemical · 394, 134356
03 / ON-SITE BIOSENSING
Biosensors and Bioelectronics · 236, 115403
04 / MATERIALS & FABRICATION
ACS Sustainable Chemistry & Engineering · 12, 4486–4496
03 / PRINCIPAL INVESTIGATOR

04 / OUR PEOPLE
顯示 6 位成員 / Showing 6 members
MASTER’S STUDENT
第一屆碩士班學生Master’s student · First cohort
MASTER’S STUDENT
第一屆碩士班學生Master’s student · First cohort
UNDERGRADUATE
實驗室專題生Undergraduate researcher
UNDERGRADUATE
實驗室專題生Undergraduate researcher
UNDERGRADUATE
實驗室專題生Undergraduate researcher
UNDERGRADUATE
實驗室專題生Undergraduate researcher
05 / TRAINING & COLLABORATION
MASTER'S TRAINING ROADMAP · IVD DEVELOPMENT
以臨床體外診斷(IVD)設備為研究主軸,從選擇檢測目標、建立微流體與生物感測方法,到裝置整合、性能評估及研究成果撰寫,規劃兩年研究路徑。Our IVD-oriented research pathway spans diagnostic target selection, microfluidics and biosensing methods, prototype integration, performance evaluation, and scientific writing.
將樣本處理與分析流程整合進微流體或微感測器;學習通道設計、晶片材料選擇、CNC/3D 列印、微奈米製程及流體控制。Miniaturize sample handling and detection through microfluidic design, material selection, fabrication, and flow control.
依臨床需求確立微生物、癌症標記、目標基因、蛋白質或小分子等分析物;探索陣列式、平行通道及數位化檢測與訊號讀出。Choose clinically relevant microbial or cancer biomarkers, genes, proteins, or small molecules, and explore arrayed, parallel, or digital assays.
建立影像判讀、資料標註與數據分析能力,循序學習 ImageJ、Python 及視需要採用 CNN、ResNet 或 YOLO;練習使用合適的開源專案與資料集。Develop image interpretation and analysis skills with ImageJ, Python, and, where appropriate, CNN/ResNet/YOLO and open-source datasets.
閱讀臨床 IVD 與相關技術文獻,選定微流體/微感測器應用情境、目標分析物及預期性能指標。建立基本操作、研究紀錄及實驗設計能力。Review IVD literature; define the clinical use case, analyte, performance targets, and core experimental skills.
開發陣列式或數位化微流體與感測器原型,進行生物辨識、訊號讀出及測試流程建立。依研究需要整合光學/電學量測、影像演算法或 DNA 電路放大等技術。Build microfluidic/biosensor prototypes; establish recognition and readout workflows, optionally using optical/electrical sensing, imaging, or DNA circuit amplification.
整合晶片、流體控制、感測及影像分析;調整關鍵參數,檢驗靈敏度、特異性、檢測極限、重複性與裝置穩定性。完成預定功能與性能指標,整理研討會資料。Integrate subsystems, optimize parameters, and evaluate sensitivity, specificity, detection limits, repeatability, stability, and predefined performance targets.
完成確認性實驗、統計分析及碩士論文。依研究成果安排學術論文投稿、臨床合作單位測試或進一步確效;涉及人體研究或可識別檢體時,依法規及合作機構要求辦理 IRB 等審查。Complete confirmatory experiments, analysis, and the thesis. Manuscripts or clinical collaborations depend on results; human-subject work follows applicable ethics approvals.
01 · 參數最佳化建立合適的對照組、測量條件及重複性驗證,據以調整晶片與分析方法。Optimize operating parameters using controls, defined conditions, and repeatability testing.
02 · 裝置達成目標依事先訂定的規格,確認功能及性能指標達到研究預期,並記錄尚待改善的限制。Verify the device against predefined functional and analytical requirements, and document limitations.
以上為預期培訓進度,依個別研究題目、實驗結果、設備條件與系所畢業規範調整。正式臨床確效、IRB、期刊接受與專利成果均依研究需要及相關審核程序辦理。This is a planning roadmap, not a guaranteed graduation schedule. Formal clinical validation, IRB approval, publications, and IP follow the project's needs and applicable processes.
06 / PUBLICATIONS
顯示 10 篇論文 / Showing 10 publications
此分類目前尚無公開論文。No publications in this category yet.
Sensors and Actuators B: Chemical · 140778
Chemical Engineering Journal · 499, 155994
Sensors and Actuators B: Chemical · 394, 134356
Biosensors and Bioelectronics · 236, 115403
ACS Sustainable Chemistry & Engineering · 12, 4486–4496
Advanced Functional Materials · 35(14), 2420439
Langmuir · 39, 1897–1904
Advanced Materials Technologies · 8, 2201026
Sensors and Actuators B: Chemical · 316, 128003
Scientific Reports · 9(1), 8145
EXPLORE THE LAB
SCIENCE & COLLABORATION
生醫微流體是在微小流道或腔室中,控制少量液體、細胞與分子的技術,應用於生物研究、檢體處理與檢測。你可以把它想成晶片上的液體路網:設計流道、閥門與反應區,讓檢體依序完成輸送、混合、分離或反應。
例如,讓細胞通過分流結構,或讓檢體與試劑在指定位置接觸,再接上感測與讀值。實際效果取決於流道設計、材料、流動條件與檢體特性。
Biomedical microfluidics controls small amounts of liquid, cells and molecules in miniature channels or chambers for biological research, sample preparation and testing. Think of a liquid-routing network on a chip: channels, valves and reaction regions guide transport, mixing, separation and reactions.
Examples include routing cells through branching channels or bringing samples and reagents together before sensing. Performance depends on geometry, materials, flow conditions and sample properties.
延伸閱讀 / Further reading: NIST · On-chip measurements ↗
感測器把溫度、壓力、光線或化學物質等變化,轉成可以量測與記錄的訊號。生醫應用會依目標選擇電流、電位、螢光、顏色或其他讀取方式。
生物感測器進一步利用酵素、抗體或核酸等生物辨識元件,結合訊號轉換機制來偵測目標。例如,酵素型血糖感測器以葡萄糖相關反應產生電化學訊號,再經校正換算濃度;微流體則能負責把檢體送到感測區。訊號是否具有足夠選擇性與準確度,需要以校正、干擾測試及實際檢體驗證。
A sensor converts a change—such as temperature, pressure, light or chemical composition—into a measurable signal. Biomedical systems may read current, potential, fluorescence, color or other outputs, depending on the target.
A biosensor combines biological recognition, such as an enzyme, antibody or nucleic acid, with signal transduction. For example, an enzyme-based glucose sensor generates an electrochemical signal that is calibrated to concentration. Microfluidics can deliver the sample to the sensing region. Selectivity and accuracy require calibration, interference testing and validation with relevant samples.
延伸閱讀 / Further reading: NIH · Sensors ↗ · IUPAC · Biosensor ↗
百洑傳息實驗室以微流體、生物感測與體外診斷為核心,網站介紹呼氣冷凝液採集、聲學微流體、離心式細菌檢測與 Au/SiC 光催化微流體等研究。
Bioflumen & Xensor Lab focuses on microfluidics, biosensing and in vitro diagnostics. Featured studies cover exhaled breath condensate collection, acoustic microfluidics, centrifugal bacterial detection and Au/SiC photocatalytic microfluidics.
請在下方聯絡表單選擇產學合作或研究合作,填寫單位、聯絡 Email,以及研究問題或合作構想。亦可直接寄信至 jimhao@nycu.edu.tw。
Select industry–academia or research collaboration in the contact form and provide your organization, email and project idea. You can also email jimhao@nycu.edu.tw.
請在聯絡表單選擇加入實驗室或學生專題,簡述目前就讀單位、研究興趣與希望參與的時間,與張鈞豪助理教授聯繫。
Select joining the lab or student project in the contact form. Introduce your current institution, research interests and intended timeframe to contact Assistant Professor Chun-Hao Chang.
歡迎醫技、生科、生醫及相關領域學生洽詢。醫技背景能幫助理解檢體、檢驗流程、品質控制與臨床需求;晶片設計、基礎製程、影像分析和程式工具可透過研究訓練循序學習。加入前建議具備基本實驗紀錄習慣、文獻閱讀意願,以及願意嘗試跨領域問題的態度。
Applicants from medical laboratory science, biotechnology, biomedical science, and related backgrounds are welcome. Clinical laboratory knowledge supports sample handling, quality control, and use-case definition. Engineering and coding skills can be developed during training.
預計利用暑假進行約 2–3 週的基礎設備與實驗流程訓練,包括實驗安全、紀錄方式、晶片或影像工具入門。碩一上確認研究問題及檢測目標,碩一下建立原型和測試方法,暑假至碩二上進行參數最佳化及整合驗證,碩二下完成論文與口試準備。兩年為研究規劃目標,實際畢業須符合校系修課與審查規定,並依個人研究進度調整。
The plan begins with approximately 2–3 weeks of summer orientation in safety, documentation, equipment, and introductory chip/imaging tools. Students then progress from target definition to prototypes, validation, and thesis preparation. Graduation timing follows research progress and degree regulations.
可能接觸微流體設計、簡易晶片製程(如 CNC、3D 列印與模具製作)、生物感測與檢驗試劑開發、訊號量測、影像處理、資料分析,以及 Python 或 AI 輔助程式開發(Vibe Coding)。若研究包含影像辨識,可進一步評估 ImageJ、CNN、ResNet、YOLO 或公開資料集;是否使用深度學習取決於研究問題、資料量與所需的驗證方式。
Depending on the project, training may include microfluidics, CNC/3D-printed prototypes, biosensing, reagents, signal acquisition, imaging, and Python/AI-assisted coding. Deep learning tools such as CNN, ResNet, or YOLO are selected when appropriate to the question and available data.
訓練包含文獻搜尋與判讀、研究假說與實驗設計、對照組與資料品質管理、專案進度追蹤、團隊協作、問題排查,以及中英文簡報、圖表製作和論文撰寫。學生需要能解釋實驗結果及其限制,並將下一步工作轉化為可執行的計畫。
Students practice literature review, hypothesis-driven experimental design, controls, data quality, project tracking, troubleshooting, teamwork, scientific presentations, figures, and manuscript writing, including clear interpretation of study limitations.
研究訓練可銜接體外診斷(IVD)與臨床檢驗設備公司、醫療器材研發、生物感測與微流體相關企業、半導體和生醫交叉領域的研發職務、研究機構,以及國內外博士班。實際職缺仍取決於個人專題、技能組合、求職條件與產業需求。具轉譯潛力的成果可另評估產學合作、專利或技術移轉。
Potential paths include IVD and laboratory equipment companies, medical-device R&D, biosensing/microfluidics, biomedical–semiconductor engineering, research institutes, and doctoral study. Opportunities depend on each graduate’s skills and the job market; suitable projects may also lead to industry collaboration or IP evaluation.
07 / CONTACT
研究討論、學生專題與跨領域合作,歡迎聯絡。For research discussions, student projects and interdisciplinary collaboration.
jimhao@nycu.edu.tw ↗